Syllabus for Chhattisgarh Health & Family Welfare Department Lab Technicians

Chhattisgarh Health & Family Welfare Department Lab Technicians Syllabus

The comprehensive syllabus for the Chhattisgarh Health & Family Welfare Department Lab Technicians’ exam generally includes topics related to laboratory technology, medical diagnostics, and healthcare practices. Below is an outline of the syllabus:

1. Basic Laboratory Techniques

Introduction to Laboratory Technology:

• Basic principles and practices in a clinical laboratory.

Laboratory Safety:

• Guidelines for safety in the laboratory, handling of hazardous materials, and waste disposal.

Laboratory Equipment:

• Usage, maintenance, and calibration of laboratory instruments like microscopes, centrifuges, spectrophotometers, etc.

Sample Collection and Handling:

• Techniques for collecting and handling blood, urine, sputum, and other biological samples.

2. Clinical Pathology

Hematology:

• Blood composition, blood cell counts, hemoglobin estimation, blood typing, and blood smear examination.

Urinalysis:

• Physical, chemical, and microscopic examination of urine, including tests for glucose, proteins, and ketones.

Stool Examination:

• Detection of parasites, ova, and occult blood in stool samples.

Body Fluids Analysis:

• Examination of cerebrospinal fluid (CSF), pleural fluid, and other body fluids.

3. Biochemistry

Clinical Biochemistry:

• Analysis of blood glucose, urea, creatinine, electrolytes, liver function tests (LFTs), and lipid profiles.

Enzymology:

• Assay of enzymes like amylase, lipase, and alkaline phosphatase.

Endocrinology:

• Tests for thyroid function, insulin, cortisol, and other hormones.

Blood Gas Analysis:

• Interpretation of arterial blood gases (ABGs) for pH, pCO2, pO2, and bicarbonate levels.

4. Microbiology

Introduction to Microbiology:

• Basics of microbiology, classification, and morphology of bacteria, viruses, fungi, and parasites.

Sterilization and Disinfection:

• Methods of sterilization, autoclaving, and disinfection techniques.

Culture Techniques:

• Preparation of culture media, inoculation, incubation, and identification of microbial growth.

Antibiotic Sensitivity Testing:

• Methods for determining the sensitivity of microorganisms to antibiotics.

5. Histopathology

Tissue Processing:

• Fixation, embedding, sectioning, and staining of tissue samples for microscopic examination.

Histological Staining:

• Principles and techniques of routine and special stains, including Hematoxylin and Eosin (H&E), PAS, and immunohistochemistry.

Cytology:

• Preparation and examination of cytology samples, including Pap smears and fine needle aspiration cytology (FNAC).

6. Immunology and Serology

Basic Immunology:

• Overview of the immune system, antigen-antibody reactions, and types of immunity.

Serological Tests:

• Techniques like ELISA, VDRL, Widal test, CRP, and tests for HIV, HBsAg, and other infectious diseases.

Blood Banking:

• Blood grouping, cross-matching, Rh typing, and preparation of blood components.

7. Molecular Biology

DNA/RNA Extraction:

• Techniques for isolating nucleic acids from clinical samples.

Polymerase Chain Reaction (PCR):

• Principles, procedure, and applications of PCR in diagnostics.

Electrophoresis:

• Techniques for separating DNA, RNA, and proteins using gel electrophoresis.

Molecular Diagnostics:

• Use of molecular methods for the detection of genetic disorders, infectious agents, and cancer markers.

8. Parasitology

Introduction to Parasitology:

• Classification and life cycles of medically important parasites.

Parasitic Infections:

• Laboratory diagnosis of malaria, filariasis, leishmaniasis, and other parasitic diseases.

Microscopic Examination:

• Identification of parasites in blood, stool, and tissue samples.

9. Virology

Introduction to Virology:

• Structure, classification, and replication of viruses.

Viral Infections:

• Laboratory diagnosis of viral infections like influenza, hepatitis, HIV, and COVID-19.

Serological and Molecular Techniques:

• Use of serological and molecular methods for virus detection.

10. Quality Control in Laboratory

Quality Assurance:

• Principles of quality control in the laboratory, internal and external quality control programs.

Standard Operating Procedures (SOPs):

• Importance of SOPs, documentation, and laboratory accreditation.

Error Management:

• Identification, analysis, and management of pre-analytical, analytical, and post-analytical errors.

11. Blood Banking and Transfusion Medicine

Blood Donation:

• Blood donor selection, blood collection, and processing.

Transfusion Reactions:

• Recognition and management of transfusion reactions.

Component Therapy:

• Preparation and use of blood components like plasma, platelets, and cryoprecipitate.

12. Laboratory Management

Laboratory Organization:

• Management of laboratory services, staffing, and workflow.

Inventory Control:

• Management of laboratory reagents, chemicals, and supplies.

Information Systems:

• Use of laboratory information systems (LIS) for data management and reporting.